Improved photon yield from a green dye with a reducing and oxidizing system

Antoine Le Gall (1) , David Dulin (1) , Gilles Clavier (2) , Rachel Méallet-Renault (2) , Philippe Bouyer (1, 3) , Karen Perronet (1) , Nathalie Westbrook (1)

1 Laboratoire Charles Fabry de l'Institut d'Optique / Otique atomique - bio
2 PPSM - Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires
3 lp2n-04,lp2n-11

An optimized chemistry turns a small green dye into a useful label for single- molecule experiments where steric hin- drance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron-dipyrromethene) FL fluorophore emits, on average, 20 times more pho- tons around 510 nm (see picture) and its lifetime before photobleaching is in- creased by the same amount, reaching several seconds.

Keywords: fluorescence; biophysics; single-molecule studies; photophysics; electron transfer

Domains: Biological Physics [physics.bio-ph]; Chemical Physics [physics.chem-ph]; Optics [physics.optics]

Antoine Le Gall, David Dulin, Gilles Clavier, Rachel Méallet-Renault, Philippe Bouyer, et al.. Improved photon yield from a green dye with a reducing and oxidizing system. ChemPhysChem, 2011, 12 (9), pp.1657-1660. ⟨10.1002/cphc.201100085⟩. ⟨hal-00597706⟩