Improved photon yield from a green dye with a reducing and oxidizing system

Abstract : An optimized chemistry turns a small green dye into a useful label for single- molecule experiments where steric hin- drance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron-dipyrromethene) FL fluorophore emits, on average, 20 times more pho- tons around 510 nm (see picture) and its lifetime before photobleaching is in- creased by the same amount, reaching several seconds.
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Antoine Le Gall, David Dulin, Gilles Clavier, Rachel Méallet-Renault, Philippe Bouyer, et al.. Improved photon yield from a green dye with a reducing and oxidizing system. ChemPhysChem, Wiley-VCH Verlag, 2011, 12 (9), pp.1657-1660. ⟨10.1002/cphc.201100085⟩. ⟨hal-00597706⟩

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