Improved photon yield from a green dye with a reducing and oxidizing system

Antoine Le Gall 1 David Dulin 1 Gilles Clavier 2 Rachel Méallet-Renault 2 Philippe Bouyer 1, 3 Karen Perronet 1 Nathalie Westbrook 1
1 Laboratoire Charles Fabry de l'Institut d'Optique / Otique atomique - bio
LCFIO - Laboratoire Charles Fabry de l'Institut d'Optique
2 PPSM - Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires
3 lp2n-04,lp2n-11
LP2N - Laboratoire Photonique, Numérique et Nanosciences
Abstract : An optimized chemistry turns a small green dye into a useful label for single- molecule experiments where steric hin- drance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron-dipyrromethene) FL fluorophore emits, on average, 20 times more pho- tons around 510 nm (see picture) and its lifetime before photobleaching is in- creased by the same amount, reaching several seconds.
Keywords : electron transfer photophysics fluorescence biophysics single-molecule studies
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Journal articles
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Submitted on : Wednesday, June 1, 2011 - 4:15:32 PM
Last modification on : Wednesday, May 2, 2018 - 4:52:01 PM
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Antoine Le Gall, David Dulin, Gilles Clavier, Rachel Méallet-Renault, Philippe Bouyer, et al.. Improved photon yield from a green dye with a reducing and oxidizing system. ChemPhysChem, Wiley-VCH Verlag, 2011, 12 (9), pp.1657-1660. ⟨10.1002/cphc.201100085⟩. ⟨hal-00597706⟩

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