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Journal Articles Biosensors and Bioelectronics Year : 2009

Quantitative analysis of enhanced light irradiance in waveguide-based fluorescent microarrays

Gabriel Sagarzazu
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Mélanie Bedu
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L. Martinelli
Nicolas Pelletier
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Viatcheslav I. Safarov
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Abstract

Probing microarray assays in the presence of a hybridization mix retrieves precious information on hybridization kinetics. However, in common detection schemes, useful surface signals compete with the high supernatant background from labelled targets in the mix. A known solution consists in exciting specifically the microarray surface with evanescent fields. Configurations using planar opticalwaveguides to produce such fields are shownhere to present also a dramatic excitation irradiance enhancement at the guide/surrounding matter interface. We compare theoretically and experimentally a guided excitation with a classical external excitation. A full electromagnetic analysis predicts an irradiance increase higher than 104 for adequately tailored waveguides.We deposited high-index TiO2 sol–gel waveguides on glass substrates according to best simulations. Quantitative enhancement analysis exploiting actual biological fluorescent spots perfectly confirms the irradiance amplification effect of a thin waveguide. The impact of amplification on the design of biochip readers is discussed since it leaves ample margin for simple and low-cost light couplers, advantageous in affordable readers and sensor systems.
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hal-00567021 , version 1 (24-08-2022)

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Gabriel Sagarzazu, Mélanie Bedu, L. Martinelli, Nicolas Pelletier, Viatcheslav I. Safarov, et al.. Quantitative analysis of enhanced light irradiance in waveguide-based fluorescent microarrays. Biosensors and Bioelectronics, 2009, 24 (7), pp.2281. ⟨10.1016/j.bios.2008.11.001⟩. ⟨hal-00567021⟩
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