Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source

Pierre Blandin (1, 2) , Sandrine Leveque-Fort (1) , S. Lecart (1) , C. Cossec (3) , Marie-Claude Potier (3) , Zsolt Lenkei (3) , Frédéric Druon (2) , Patrick Georges (2)

1 PPM - Laboratoire de Photophysique Moléculaire
2 Laboratoire Charles Fabry de l'Institut d'Optique / Elsa
3 NDC - Neurobiologie et diversité cellulaire

We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide- field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.

Domains: Optics [physics.optics]

Pierre Blandin, Sandrine Leveque-Fort, S. Lecart, C. Cossec, Marie-Claude Potier, et al.. Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source. Applied optics, 2009, 48 (3), pp.553-559. ⟨hal-00533147⟩