Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source
Résumé
We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide- field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
Domaines
Optique [physics.optics]
Origine : Fichiers éditeurs autorisés sur une archive ouverte
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