Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source - Archive ouverte HAL Access content directly
Journal Articles Applied optics Year : 2009

Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source

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Abstract

We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide- field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
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Dates and versions

hal-00533147 , version 1 (30-03-2012)

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  • HAL Id : hal-00533147 , version 1

Cite

Pierre Blandin, Sandrine Leveque-Fort, S. Lecart, C. Cossec, Marie-Claude Potier, et al.. Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source. Applied optics, 2009, 48 (3), pp.553-559. ⟨hal-00533147⟩
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