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Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source

Abstract : We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide- field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
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https://hal-iogs.archives-ouvertes.fr/hal-00533147
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Submitted on : Friday, March 30, 2012 - 5:53:55 PM
Last modification on : Wednesday, September 23, 2020 - 11:55:01 AM
Long-term archiving on: : Tuesday, December 13, 2016 - 5:34:25 PM

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Pierre Blandin, Sandrine Leveque-Fort, S. Lecart, C. Cossec, Marie-Claude Potier, et al.. Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source. Applied optics, Optical Society of America, 2009, 48 (3), pp.553-559. ⟨hal-00533147⟩

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