Determination of scattering coefficient and scattering anisotropy factor of tissue-mimicking phantoms using line-field confocal optical coherence tomography (LC-OCT) - Groupe Biophotonique Access content directly
Journal Articles Journal of the European Optical Society : Rapid publications Year : 2023

Determination of scattering coefficient and scattering anisotropy factor of tissue-mimicking phantoms using line-field confocal optical coherence tomography (LC-OCT)

Léna Waszczuk
Jonas Ogien
  • Function : Author
Frederic Pain
  • Function : Author
  • PersonId : 741417
  • IdHAL : fpain
Arnaud Dubois

Abstract

Line-field Confocal Optical Coherence Tomography (LC-OCT) is an imaging modality based on a combination of time-domain optical coherence tomography and reflectance confocal microscopy. LC-OCT provides three-dimensional images of semi-transparent samples with a spatial resolution of ∼1 μm. The technique is primarily applied to in vivo skin imaging. The image contrast in LC-OCT arises from the backscattering of incident light by the sample microstructures, which is determined by the optical scattering properties of the sample, characterized by the scattering coefficient μ s and the scattering anisotropy factor g . In biological tissues, the scattering properties are determined by the organization, structure and refractive indexes of the sample. The measurement of these properties using LC-OCT would therefore allow a quantitative characterization of tissues in vivo . We present a method for extracting the two scattering properties μ s and g of tissue-mimicking phantoms from 3D LC-OCT images. The method provides the mean values of μ s and g over a lateral field of view of 1.2 mm × 0.5 mm ( x × y ). It can be applied to monolayered and bilayered samples, where it allows extraction of μ s and g of each layer. Our approach is based on a calibration using a phantom with known optical scattering properties and on the application of a theoretical model to the intensity depth profiles acquired by LC-OCT. It was experimentally tested against integrating spheres and collimated transmission measurements for a set of monolayered and bilayered scattering phantoms.
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Dates and versions

hal-04282160 , version 1 (13-11-2023)

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Léna Waszczuk, Jonas Ogien, Frederic Pain, Arnaud Dubois. Determination of scattering coefficient and scattering anisotropy factor of tissue-mimicking phantoms using line-field confocal optical coherence tomography (LC-OCT). Journal of the European Optical Society : Rapid publications, 2023, 19 (2), pp.39. ⟨10.1051/jeos/2023037⟩. ⟨hal-04282160⟩
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