Determination of scattering coefficient and scattering anisotropy factor of tissue-mimicking phantoms using line-field confocal optical coherence tomography (LC-OCT)

Léna Waszczuk (1) , Jonas Ogien (1) , Frederic Pain (2) , Arnaud Dubois (2)

1 DAMAE - DAMAE Medical
2 Laboratoire Charles Fabry / Biophotonique

Line-field Confocal Optical Coherence Tomography (LC-OCT) is an imaging modality based on a combination of time-domain optical coherence tomography and reflectance confocal microscopy. LC-OCT provides three-dimensional images of semi-transparent samples with a spatial resolution of ∼1 μm. The technique is primarily applied to in vivo skin imaging. The image contrast in LC-OCT arises from the backscattering of incident light by the sample microstructures, which is determined by the optical scattering properties of the sample, characterized by the scattering coefficient μ s and the scattering anisotropy factor g . In biological tissues, the scattering properties are determined by the organization, structure and refractive indexes of the sample. The measurement of these properties using LC-OCT would therefore allow a quantitative characterization of tissues in vivo . We present a method for extracting the two scattering properties μ s and g of tissue-mimicking phantoms from 3D LC-OCT images. The method provides the mean values of μ s and g over a lateral field of view of 1.2 mm × 0.5 mm ( x × y ). It can be applied to monolayered and bilayered samples, where it allows extraction of μ s and g of each layer. Our approach is based on a calibration using a phantom with known optical scattering properties and on the application of a theoretical model to the intensity depth profiles acquired by LC-OCT. It was experimentally tested against integrating spheres and collimated transmission measurements for a set of monolayered and bilayered scattering phantoms.

Mots clés: Line-field confocal optical coherence tomography Optical coherence tomography Reflectance confocal microscopy Optical properties Scattering; Line-field confocal optical coherence tomography; Optical coherence tomography; Reflectance confocal microscopy; Optical properties; Scattering

Domaines: Physique [physics]

Léna Waszczuk, Jonas Ogien, Frederic Pain, Arnaud Dubois. Determination of scattering coefficient and scattering anisotropy factor of tissue-mimicking phantoms using line-field confocal optical coherence tomography (LC-OCT). Journal of the European Optical Society : Rapid publications, 2023, 19 (2), pp.39. ⟨10.1051/jeos/2023037⟩. ⟨hal-04282160⟩