Line‐field confocal optical coherence tomography as a tool for three‐dimensional in vivo quantification of healthy epidermis: A pilot study - Institut d'Optique Graduate School Access content directly
Journal Articles Journal of Biophotonics Year : 2022

Line‐field confocal optical coherence tomography as a tool for three‐dimensional in vivo quantification of healthy epidermis: A pilot study

Julie Chauvel-Picard
  • Function : Author
Vincent Bérot
  • Function : Author
Linda Tognetti
  • Function : Author
Carmen Orte Cano
  • Function : Author
Margot Fontaine
  • Function : Author
Clément Lenoir
  • Function : Author
Javiera Pérez-Anker
  • Function : Author
Susana Puig
  • Function : Author
Arnaud Dubois
Sandra Forestier
  • Function : Author
Jilliana Monnier
  • Function : Author
Randa Jdid
  • Function : Author
Gabriel Cazorla
  • Function : Author
Mélanie Pedrazzani
Antoine Sanchez
  • Function : Author
Pietro Rubegni
  • Function : Author
Véronique del Marmol
  • Function : Author
Joseph Malvehy
  • Function : Author
Elisa Cinotti
Jean Perrot
  • Function : Author
Mariano Suppa
  • Function : Author

Abstract

Abstract Epidermal three‐dimensional (3D) topography/quantification has not been completely characterized yet. The recently developed line‐field confocal optical coherence tomography (LC‐OCT) provides real‐time, high‐resolution, in‐vivo 3D imaging of the skin. This pilot study aimed at quantifying epidermal metrics (epidermal thicknesses, dermal‐epidermal junction [DEJ] undulation and keratinocyte number/shape/size) using 3D LC‐OCT. For each study participant (8 female, skin‐type‐II, younger/older volunteers), seven body sites were imaged with LC‐OCT. Epidermal metrics were calculated by segmentations and measurements assisted by artificial intelligence (AI) when appropriate. Thicknesses of epidermis/SC, DEJ undulation and keratinocyte nuclei volume varied across body sites. Evidence of keratinocyte maturation was observed in vivo: keratinocyte nuclei being small/spherical near the DEJ and flatter/elliptical near the skin surface. Skin microanatomy can be quantified by combining LC‐OCT and AI. This technology could be highly relevant to understand aging processes and conditions linked to epidermal disorders. Future clinical/research applications are to be expected in this scenario.
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Dates and versions

hal-04282333 , version 1 (13-11-2023)

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Julie Chauvel-Picard, Vincent Bérot, Linda Tognetti, Carmen Orte Cano, Margot Fontaine, et al.. Line‐field confocal optical coherence tomography as a tool for three‐dimensional in vivo quantification of healthy epidermis: A pilot study. Journal of Biophotonics, 2022, 15 (2), ⟨10.1002/jbio.202100236⟩. ⟨hal-04282333⟩
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