Dual-color Three-photon Microscopy for Deep Imaging of Neural Tissue

Lamiae Abdeladim (1) , Khmaies Guesmi (2) , Pierre Mahou (1) , Samuel Tozer (3) , Takuma Kumamoto (3) , Jùlia Ferrer-Ortas (1) , Nicolas Dray (4) , Karine Loulier (3) , Marc Hanna (2) , Patrick Georges (2) , Jean Livet (3) , Willy Supatto (1) , Frédéric Druon (2) , Emmanuel Beaurepaire (1)

1 LOB - Laboratoire d'Optique et Biosciences
2 Laboratoire Charles Fabry / Lasers
3 Institut de la Vision
4 BGMCD - Bases Génétiques, Moléculaires et Cellulaires du Développement

We demonstrate dual-color three-photon imaging of red and green fluorescent proteins in neural tissue using a novel ultrafast laser design providing simultaneous excitation at 1300 and 1700 nm.

Domains: Optics [physics.optics]

Lamiae Abdeladim, Khmaies Guesmi, Pierre Mahou, Samuel Tozer, Takuma Kumamoto, et al.. Dual-color Three-photon Microscopy for Deep Imaging of Neural Tissue. Biomedical Optics Congress 2018, Apr 2018, Hollywood, United States. OSA, 2018, ⟨10.1364/TRANSLATIONAL.2018.JW3A.62⟩. ⟨hal-01810035⟩