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Journal Articles Sensors and Actuators B: Chemical Year : 2015

Compact 5-LEDs illumination system for multi-spectral surface plasmon resonance sensing

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Abstract

In this contribution, we quantify the performances of a low-cost, compact, multi-spectral illumination system allowing spectral interrogation to be performed in surface plasmon resonance imaging (SPRI) systems. Previous work demonstrated the feasibility of spectral interrogation based on a discrete number of scanning wavelengths using a white source and monochromator. Here, we expand this concept by building a multi-spectral SPRI system with five light-emitting diodes (LEDs). The development focuses on the improvement of the illumination stability, which is a critical parameter for the precision of the spectral interrogation measurement. The obtained prototype exhibits sensitivity and resolution properties comparable with reflectivity-interrogation-based SPRI systems, with the advantages of spectral interrogation, i.e. increased dynamical range and robustness towards solution index variations. These performances, as well as the real-time and 2-dimensional imaging modalities of the five-LEDs setup, are illustrated by a standard experiment of DNA–DNA interaction monitoring on a biochip format.
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Dates and versions

hal-01230282 , version 1 (27-11-2015)

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Alexandra Sereda, Julien Moreau, Marine Boulade, Aurore Olivéro, Michael Canva, et al.. Compact 5-LEDs illumination system for multi-spectral surface plasmon resonance sensing. Sensors and Actuators B: Chemical, 2015, 209, pp.208-211. ⟨10.1016/j.snb.2014.11.080⟩. ⟨hal-01230282⟩
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