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Conference Papers Year : 2014

Using Optical Lattice for STED Parallelization

Bin Yang
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  • PersonId : 960220
Jean-Baptiste Trebbia
Brahim Lounis
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  • PersonId : 941315

Abstract

Being a scanning microscopy, Stimulated Emission Depletion (STED) needs to be parallelized for fast wide-field imaging. Here, we achieve large parallelization of STED microscopy using well-designed Optical Lattice (OL) for depletion, together with a fast camera for detection. Depletion optical lattices with 100 intensity "zeros" are generated by four-beam interference. Scanning only a unit cell, as small as 290 nm by 290 nm, of the depletion OL is sufficient for STED imaging. The OL-STED microscopy acquires super-resolution images with 70 nm resolution and at a rate of 12.5 frames per image.
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Dates and versions

hal-01066165 , version 1 (19-09-2014)

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Bin Yang, Jean-Baptiste Trebbia, Brahim Lounis. Using Optical Lattice for STED Parallelization. SPIE Optics+Photonics, Aug 2014, San Diego, United States. pp.91690E, ⟨10.1117/12.2064820⟩. ⟨hal-01066165⟩
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