Using Optical Lattice for STED Parallelization

Bin Yang 1 Jean-Baptiste Trebbia 1 Brahim Lounis 1
1 lp2n-01,lp2n-12
LP2N - Laboratoire Photonique, Numérique et Nanosciences
Abstract : Being a scanning microscopy, Stimulated Emission Depletion (STED) needs to be parallelized for fast wide-field imaging. Here, we achieve large parallelization of STED microscopy using well-designed Optical Lattice (OL) for depletion, together with a fast camera for detection. Depletion optical lattices with 100 intensity "zeros" are generated by four-beam interference. Scanning only a unit cell, as small as 290 nm by 290 nm, of the depletion OL is sufficient for STED imaging. The OL-STED microscopy acquires super-resolution images with 70 nm resolution and at a rate of 12.5 frames per image.
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Conference papers
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https://hal-iogs.archives-ouvertes.fr/hal-01066165
Contributor : Bin Yang <>
Submitted on : Friday, September 19, 2014 - 12:06:28 PM
Last modification on : Thursday, January 11, 2018 - 6:26:24 AM

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Bin Yang, Jean-Baptiste Trebbia, Brahim Lounis. Using Optical Lattice for STED Parallelization. SPIE Optics+Photonics, Aug 2014, San Diego, United States. pp.91690E, ⟨10.1117/12.2064820⟩. ⟨hal-01066165⟩

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