Sensor-integrated fluorescent microarray for ultrahigh sensitivity direct-imaging bioassays: Role of a high rejection of excitation light

Lucio Martinelli (1) , Houtai Choumane (1) , Khoi-Nguyen Ha (1) , Gabriel Sagarzazu (1) , Carole Goutel (1) , Claude Weisbuch (1) , Thierry Gacoin (2) , Henri Benisty (3)

1 Genewave
2 LPMC - Laboratoire de physique de la matière condensée
3 Laboratoire Charles Fabry de l'Institut d'Optique / Naphel

Fluorescent microarrays exploit fluorescent labeled targets bound to immobilized biomolecular probes. Their signal-to-noise ratio is limited by the collection aperture in common confocal geometries. Taking advantage of a very high rejection filter deposited onto a silicon arrayed detector (coupled-charge device or complementary metal-oxide semiconductor), it is demonstrated that a highly compact lens-free assay with photon collection of order unity operates with a 30-fold improvement over a conventional (substrate + free-space optics) scheme. Through analysis of improvements over the present demonstrator, a single molecule per pixel sensitivity is predicted.

Domains: Optics [physics.optics]

Lucio Martinelli, Houtai Choumane, Khoi-Nguyen Ha, Gabriel Sagarzazu, Carole Goutel, et al.. Sensor-integrated fluorescent microarray for ultrahigh sensitivity direct-imaging bioassays: Role of a high rejection of excitation light. Applied Physics Letters, 2007, 91, pp.083901. ⟨10.1063/1.2767209⟩. ⟨hal-00875750⟩