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Conference papers
Towards STED microscopy with nanometric optical sectioning (Orale)
Siddharth Sivankutty
1
Guillaume Dupuis
1
Christophe Lefumeux
1
Céline Mayet
1
Arnaud Dubois
2
C. Marquer
3
Sandrine Lecart
1
Marie-Claude Potier
3
Emmanuel Fort
Sandrine Leveque-Fort
4
Siddharth Sivankutty 1
Author
Guillaume Dupuis 1
Author IdHAL : guillaume-dupuis ORCID : https://orcid.org/0000-0001-7515-5718
Christophe Lefumeux 1
Author
Céline Mayet 1
Author
Arnaud Dubois 2
Author IdHAL : arnaud-dubois
C. Marquer 3
Author
Sandrine Lecart 1
Author
Marie-Claude Potier 3
Author
Emmanuel Fort
Author
Sandrine Leveque-Fort 4
Author
1
CPBM - Centre de Photonique Biomédicale (Université Paris Sud Bat 106 Arrière 91405 ORSAY - France)
- Centre Laser Université Paris Sud (France)
2
Laboratoire Charles Fabry / Biophotonique (France)
- LCF - Laboratoire Charles Fabry (2 avenue Augustin Fresnel, 91127 Palaiseau Cedex - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8501 (France)
- IOGS - Institut d'Optique Graduate School (2 avenue Augustin Fresnel, 91127 Palaiseau Cedex - France)
3
CRICM - Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (GH Pitié-Salpêtrière 47, boulevard de l'Hôpital 75651 Paris cedex 13 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7225 (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U975 (101, rue de Tolbiac, 75013 Paris - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
4
ISMO - Institut des Sciences Moléculaires d'Orsay (Université Paris-Sud 91405 Orsay cedex - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8214 (France)
Abstract : Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an alternative technique for optically sectioning fluorescent emitters close to the water-glass interface by selectively filtering the supercritical emission at the pupil plane. We discuss the instrumental development of such a system and its combination with other imaging techniques.
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Conference papers
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Complete list of metadatas
https://hal-iogs.archives-ouvertes.fr/hal-00835893
Contributor : Arnaud Dubois <>
Submitted on : Thursday, June 20, 2013 - 9:33:37 AM
Last modification on : Wednesday, September 16, 2020 - 5:40:29 PM
Contributor : Arnaud Dubois <>
Submitted on : Thursday, June 20, 2013 - 9:33:37 AM
Last modification on : Wednesday, September 16, 2020 - 5:40:29 PM
Identifiers
- HAL Id : hal-00835893, version 1
- DOI : 10.1117/12.2004883
Collections
CNRS | PARISTECH | IOGS | ICM | UPMC | JYB-LAB | SORBONNE-UNIVERSITE | SU-MEDECINE | LCF_BIOPHOTONIQUE | LCFIO
Citation
Siddharth Sivankutty, Guillaume Dupuis, Christophe Lefumeux, Céline Mayet, Arnaud Dubois, et al.. Towards STED microscopy with nanometric optical sectioning (Orale). Photonics West - Biomedical Optics, Single molecule spectroscopy and superresolution imaging VI, Feb 2013, San Francisco, United States. pp.859018, ⟨10.1117/12.2004883⟩. ⟨hal-00835893⟩
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