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Conference papers
Towards STED microscopy with nanometric optical sectioning (Orale)
Siddharth Sivankutty
1
Guillaume Dupuis
1
Christophe Lefumeux
1
Céline Mayet
1
Arnaud Dubois
2
C. Marquer
3
Sandrine Lecart
1
Marie-Claude Potier
3
Emmanuel Fort
Sandrine Leveque-Fort
4
Siddharth Sivankutty 1
Author IdHAL : siddharth-sivankutty
Guillaume Dupuis 1
Author IdHAL : guillaume-dupuis ORCID : https://orcid.org/0000-0001-7515-5718
Christophe Lefumeux 1
Author
Céline Mayet 1
Author
Arnaud Dubois 2
Author IdHAL : arnaud-dubois
C. Marquer 3
Author
Sandrine Lecart 1
Author
Marie-Claude Potier 3
Author
Emmanuel Fort
Author
Sandrine Leveque-Fort 4
Author IdHAL : sandrine-leveque-fort ORCID : https://orcid.org/0000-0002-9218-3363
1
CPBM - Centre de Photonique Biomédicale (Université Paris Sud Bat 106 Arrière 91405 ORSAY - France)
- CLUPS - Centre Laser de l'Université Paris Sud (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
2
Laboratoire Charles Fabry / Biophotonique (France)
- LCF - Laboratoire Charles Fabry (2 avenue Augustin Fresnel, 91127 Palaiseau Cedex - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- IOGS - Institut d'Optique Graduate School (2 avenue Augustin Fresnel, 91127 Palaiseau Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8501 (France)
3
CRICM - Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (GH Pitié-Salpêtrière 47, boulevard de l'Hôpital 75651 Paris cedex 13 - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U975 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7225 (France)
4
ISMO - Institut des Sciences Moléculaires d'Orsay (Université Paris-Sud 91405 Orsay cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8214 (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
Abstract : Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an alternative technique for optically sectioning fluorescent emitters close to the water-glass interface by selectively filtering the supercritical emission at the pupil plane. We discuss the instrumental development of such a system and its combination with other imaging techniques.
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Conference papers
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https://hal-iogs.archives-ouvertes.fr/hal-00835893
Contributor : Arnaud Dubois Connect in order to contact the contributor
Submitted on : Thursday, June 20, 2013 - 9:33:37 AM
Last modification on : Thursday, May 19, 2022 - 9:32:05 AM
Contributor : Arnaud Dubois Connect in order to contact the contributor
Submitted on : Thursday, June 20, 2013 - 9:33:37 AM
Last modification on : Thursday, May 19, 2022 - 9:32:05 AM
Identifiers
- HAL Id : hal-00835893, version 1
- DOI : 10.1117/12.2004883
Collections
CNRS | IOGS | PARISTECH | ICM | UPMC | JYB-LAB | SORBONNE-UNIVERSITE | SU-MEDECINE | LCF_BIOPHOTONIQUE | LCFIO | UNIV-PARIS-SACLAY
Citation
Siddharth Sivankutty, Guillaume Dupuis, Christophe Lefumeux, Céline Mayet, Arnaud Dubois, et al.. Towards STED microscopy with nanometric optical sectioning (Orale). Photonics West - Biomedical Optics, Single molecule spectroscopy and superresolution imaging VI, Feb 2013, San Francisco, United States. pp.859018, ⟨10.1117/12.2004883⟩. ⟨hal-00835893⟩
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