Dynamic force spectroscopy on multiple bonds: Experiments and model

Thorsten Erdmann 1 Sébastien Pierrat 2 Pierre Nassoy 2 Ulrich Schwarz 1, *
* Auteur correspondant
1 University of Heidelberg - Bioquant
2 PCC - Physico-Chimie-Curie
Abstract : We probe the dynamic strength of multiple biotin-streptavidin adhesion bonds under linear loading using the biomembrane force probe setup for dynamic force spectroscopy. Measured rupture force histograms are compared to results from a master equation model for the stochastic dynamics of bond rupture under load. This allows us to extract the distribution of the number of initially closed bonds. We also extract the molecular parameters of the adhesion bonds, in good agreement with earlier results from single-bond experiments. Our analysis shows that the peaks in the measured histograms are not simple multiples of the single-bond values, but follow from a superposition procedure which generates different peak positions.
Keywords : ADHESION CLUSTERS MOLECULAR-BONDS CELL-ADHESION RECEPTOR STRENGTH IMPACT
Type de document :
Article dans une revue
EPL - Europhysics Letters, European Physical Society/EDP Sciences/Società Italiana di Fisica/IOP Publishing, 2008, 81 (4), pp.48001. 〈10.1209/0295-5075/81/48001〉
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Contributeur : Pierre Nassoy <>
Soumis le : jeudi 9 mai 2013 - 12:43:32
Dernière modification le : vendredi 31 août 2018 - 09:18:24

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Thorsten Erdmann, Sébastien Pierrat, Pierre Nassoy, Ulrich Schwarz. Dynamic force spectroscopy on multiple bonds: Experiments and model. EPL - Europhysics Letters, European Physical Society/EDP Sciences/Società Italiana di Fisica/IOP Publishing, 2008, 81 (4), pp.48001. 〈10.1209/0295-5075/81/48001〉. 〈hal-00821360〉

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