Dynamic instability of the intracellular pressure drives bleb-based motility

Benoît Maugis 1 Jan Brugués 2 Pierre Nassoy 1 Nancy Guillen 3 Pierre Sens 2 François Amblard 1, *
* Auteur correspondant
1 PCC - Physico-Chimie-Curie
2 LPCT - Laboratoire de Physico-Chimie Théorique
3 Biologie Cellulaire du Parasitisme
Abstract : We have demonstrated that the two-and three-dimensional motility of the human pathogenic parasite Entamoeba histolytica (Eh) depends on sustained instability of the intracellular hydrostatic pressure. This instability drives the cyclic generation and healing of membrane blebs, with typical protrusion velocities of 10-20 mu m/second over a few hundred milliseconds and healing times of 10 seconds. The use of a novel micro-electroporation method to control the intracellular pressure enabled us to develop a qualitative model with three parameters: the rate of the myosin-driven internal pressure increase; the critical disjunction stress of membrane-cytoskeleton bonds; and the turnover time of the F-actin cortex. Although blebs occur randomly in space and irregularly time, they can be forced to occur with a defined periodicity in confined geometries, thus confirming our model. Given the highly efficient bleb-based motility of Eh in vitro and in vivo, Eh cells represent a unique model for studying the physical and biological aspects of amoeboid versus mesenchymal motility in two-and three-dimensional environments.
Keywords : CTIN-BASED MOTILITY TUMOR-CELL INVASION MYOSIN LIGHT-CHAIN ENTAMOEBA-HISTOLYTICA CARCINOSARCOMA CELLS PLASMA-MEMBRANE CORTICAL ACTIN MIGRATION PHOSPHORYLATION MECHANISMS
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Article dans une revue
Journal of Cell Science, Company of Biologists, 2010, 123 (22), pp.3884-3892. 〈10.1242/jcs.065672〉
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https://hal-iogs.archives-ouvertes.fr/hal-00821337
Contributeur : Pierre Nassoy <>
Soumis le : jeudi 9 mai 2013 - 10:53:20
Dernière modification le : lundi 10 décembre 2018 - 01:20:16

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PASTEUR | LPCT | ESPCI | CURIE | FNCLCC | IOGS | PARISTECH | UPMC | PCC_UMR168 | INC-CNRS | PSL | SORBONNE-UNIVERSITE

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Benoît Maugis, Jan Brugués, Pierre Nassoy, Nancy Guillen, Pierre Sens, et al.. Dynamic instability of the intracellular pressure drives bleb-based motility. Journal of Cell Science, Company of Biologists, 2010, 123 (22), pp.3884-3892. 〈10.1242/jcs.065672〉. 〈hal-00821337〉

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