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Journal Articles Cell Year : 2011

Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae

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Bidisha Sinha
  • Function : Author
  • PersonId : 941108
Darius Koester
  • Function : Author
  • PersonId : 941104
Richard Ruez
  • Function : Author
  • PersonId : 941109
Pauline Gonnord
  • Function : Author
  • PersonId : 941110
Ludger Johannes
  • Function : Author
  • PersonId : 846967
Graça Raposo
  • Function : Author
  • PersonId : 834693
Pierre Sens

Abstract

The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin-and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin-and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.

Dates and versions

hal-00821331 , version 1 (09-05-2013)

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Bidisha Sinha, Darius Koester, Richard Ruez, Pauline Gonnord, Michele Bastiani, et al.. Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae. Cell, 2011, 144 (3), pp.402-413. ⟨10.1016/j.cell.2010.12.031⟩. ⟨hal-00821331⟩
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