Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae - Institut d'Optique Graduate School Access content directly
Journal Articles Cell Year : 2011

Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae

Bidisha Sinha
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  • PersonId : 941108
Darius Koester
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  • PersonId : 941104
Richard Ruez
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  • PersonId : 941109
Pauline Gonnord
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  • PersonId : 941110
Ludger Johannes
Graça Raposo
Pierre Sens

Abstract

The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin-and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin-and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.

Dates and versions

hal-00821331 , version 1 (09-05-2013)

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Bidisha Sinha, Darius Koester, Richard Ruez, Pauline Gonnord, Michele Bastiani, et al.. Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae. Cell, 2011, 144 (3), pp.402-413. ⟨10.1016/j.cell.2010.12.031⟩. ⟨hal-00821331⟩
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