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Journal articles
Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae
Bidisha Sinha
1
Darius Koester
1
Richard Ruez
2
Pauline Gonnord
2
Michele Bastiani
3
Daniel Abankawa
4
Radu Stan
5
Gillian Butler-Browne
6
Benoît Vedie
7
Ludger Johannes
2
Nobuhiro Morone
8
Robert Parton
9
Graça Raposo
2
Pierre Sens
10
Christophe Lamaze
2, *
Pierre Nassoy
11, 1, *
*
Corresponding author
Michele Bastiani 3
Author
Daniel Abankawa 4
Author
Radu V. Stan 5
Author
Gillian Butler-Browne 6
Author
Graça Raposo 2
Author
Pierre Sens 10
Author IdHAL : pierresens
Christophe Lamaze 2
Correspondent author
Pierre Nassoy 11, 1
Correspondent author
1
PCC - Physico-Chimie-Curie (Bâtiment Curie 26 rue d'Ulm 75248 PARIS CEDEX 05 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR168 (France)
- Institut Curie [Paris] ( 26 rue d'Ulm - 75248 Paris - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- INC - Institut de Chimie du CNRS (3 Rue Michel-Ange 75 016 Paris - France)
2
CDC - Compartimentation et dynamique cellulaires (26 Rue d'Ulm 75248 PARIS CEDEX 05 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR144 (France)
- Institut Curie [Paris] ( 26 rue d'Ulm - 75248 Paris - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
3
Center for Microscopy and Microanalysis - University of Queensland (Australia)
- University of Queensland [Brisbane] (Brisbane, St Lucia, QLD 4072 - Australia)
4
Center for Microscopy and Microanalysis - University of Queensland (France)
- University of Queensland [Brisbane] (Brisbane, St Lucia, QLD 4072 - Australia)
6
Thérapie des maladies du muscle strié (105 Bd de L'Hôpital 75013 Paris - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U974 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7215 (France)
8
Department of Ultrastructural Research (Japan)
9
Institute for Molecular Bioscience (Center for Microscopy and Microanalysis, and School of Biomedical Sciences, Queensland 4072 - Australia)
- University of Queensland [Brisbane] (Brisbane, St Lucia, QLD 4072 - Australia)
10
LPCT - Laboratoire de Physico-Chimie Théorique (ESPCI - 10 rue Vauquelin - 75231 Paris cedex 05 - France)
- CNRS - Centre National de la Recherche Scientifique : ESA7083 / URA1382 (France)
- ESPCI Paris - Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (10 rue Vauquelin, 75231 Paris cedex 05 - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
11
lp2n-04,lp2n-16 (France)
- LP2N - Laboratoire Photonique, Numérique et Nanosciences (Université Bordeaux 1, Bat A30, 351 cours de la Libération, 33405 TALENCE Cedex - France)
- Université Sciences et Technologies - Bordeaux 1 (351 cours de la Libération - 33405 Talence cedex - France)
- IOGS - Institut d'Optique Graduate School (2 avenue Augustin Fresnel, 91127 Palaiseau Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5298 (France)
Abstract : The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin-and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin-and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.
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Journal articles
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https://hal-iogs.archives-ouvertes.fr/hal-00821331
Contributor : Pierre Nassoy Connect in order to contact the contributor
Submitted on : Thursday, May 9, 2013 - 10:43:11 AM
Last modification on : Monday, March 14, 2022 - 11:28:02 AM
Contributor : Pierre Nassoy Connect in order to contact the contributor
Submitted on : Thursday, May 9, 2013 - 10:43:11 AM
Last modification on : Monday, March 14, 2022 - 11:28:02 AM
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- HAL Id : hal-00821331, version 1
- DOI : 10.1016/j.cell.2010.12.031
- PUBMEDCENTRAL : PMC3042189
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Bidisha Sinha, Darius Koester, Richard Ruez, Pauline Gonnord, Michele Bastiani, et al.. Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae. Cell, Elsevier, 2011, 144 (3), pp.402-413. ⟨10.1016/j.cell.2010.12.031⟩. ⟨hal-00821331⟩
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