Microscopie à fluorescence et tomographie par cohérence optique en plein champ

Abstract : Full-field optical coherence microscopy (FF-OCM) and optically-sectioned fluorescence microscopy are implemented simultaneously in a dual modality imaging system. Both modalities use a broad field illumination and acquire the entire field of view without raster scanning. Optical sectioning is achieved owing to the coherence gating property of light for FF-OCM, and structured illumination for fluorescence microscopy. The dual modality system provides complementary images in the context of biological tissue screening. FF-OCM shows the tissue micro-architecture, while fluorescence microscopy highlights specific tissue features with cellular-level resolution by using targeting contrast agents. Complementary tissue morphology and biochemical features could potentially improve the understanding of cellular functions and disease diagnosis.