Microscopie à fluorescence et tomographie par cohérence optique en plein champ

Houssine Makhlouf (1) , Karen Perronet (1) , Guillaume Dupuis (2) , Sandrine Leveque-Fort (3) , Arnaud Dubois (1)

1 Laboratoire Charles Fabry / Biophotonique
2 CPBM - Centre de Photonique Biomédicale
3 ISMO - Institut des Sciences Moléculaires d'Orsay

Full-field optical coherence microscopy (FF-OCM) and optically-sectioned fluorescence microscopy are implemented simultaneously in a dual modality imaging system. Both modalities use a broad field illumination and acquire the entire field of view without raster scanning. Optical sectioning is achieved owing to the coherence gating property of light for FF-OCM, and structured illumination for fluorescence microscopy. The dual modality system provides complementary images in the context of biological tissue screening. FF-OCM shows the tissue micro-architecture, while fluorescence microscopy highlights specific tissue features with cellular-level resolution by using targeting contrast agents. Complementary tissue morphology and biochemical features could potentially improve the understanding of cellular functions and disease diagnosis.

Domaines: Biophysique [physics.bio-ph]; Optique [physics.optics]; Instrumentations et Détecteurs [physics.ins-det]

Houssine Makhlouf, Karen Perronet, Guillaume Dupuis, Sandrine Leveque-Fort, Arnaud Dubois. Microscopie à fluorescence et tomographie par cohérence optique en plein champ. OptDiag 2012 Diagnostic et Imagerie Optique en Médecine et Biologie Biophotonique, May 2012, Paris, France. pp.000. ⟨hal-00702695⟩