Fluorescence lifetime imaging with a multifocal two photon microscope

Sandrine Leveque-Fort (1) , M.P. Fontaine-Aupart (1) , Gérard Roger (2) , Patrick Georges (2)

1 PPM - Laboratoire de Photophysique Moléculaire
2 Laboratoire Charles Fabry de l'Institut d'Optique / Elsa

Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for f luorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of f luorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample

Domains: Physics [physics]; Physics [physics]; Optics [physics.optics]

Sandrine Leveque-Fort, M.P. Fontaine-Aupart, Gérard Roger, Patrick Georges. Fluorescence lifetime imaging with a multifocal two photon microscope. Optics Letters, 2004, 29 (24), pp.2884-2886. ⟨hal-00700755⟩