Abstract : Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for f luorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of f luorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample
https://hal-iogs.archives-ouvertes.fr/hal-00700755
Contributor : Marie-Laure Edwards
<>
Submitted on : Wednesday, May 23, 2012 - 5:24:01 PM
Last modification on : Thursday, January 11, 2018 - 6:26:15 AM
Long-term archiving on : Thursday, December 15, 2016 - 9:04:36 AM
Sandrine Leveque-Fort, M.P. Fontaine-Aupart, Gérard Roger, Patrick Georges. Fluorescence lifetime imaging with a multifocal two photon microscope. Optics Letters, Optical Society of America, 2004, 29 (24), pp.2884-2886. ⟨hal-00700755⟩
