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Fluorescence lifetime imaging with a multifocal two photon microscope

Abstract : Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for f luorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of f luorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample
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Sandrine Leveque-Fort, M.P. Fontaine-Aupart, Gérard Roger, Patrick Georges. Fluorescence lifetime imaging with a multifocal two photon microscope. Optics Letters, Optical Society of America, 2004, 29 (24), pp.2884-2886. ⟨hal-00700755⟩



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