Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells

Ariane Deniset-Besseau 1, 2 Sandrine Leveque-Fort 1, 2 M.P. Fontaine-Aupart 1, 2 Gérard Roger 3 Patrick Georges 3
1 PPM - Laboratoire de Photophysique Moléculaire
2 CPBM - Centre de Photonique Biomédicale
3 Laboratoire Charles Fabry de l'Institut d'Optique / Elsa
LCFIO - Laboratoire Charles Fabry de l'Institut d'Optique
Abstract : Two-photon fluorescence microscopy is widely applied to biology and medicine to study both the structure and dynamic processes in living cells. The main issue is the slow acquisition rate due to the point scanning approach limiting the multimodal detection _x, y, z, t_. To extend the performances of this powerful technique, we present a time-resolved multifocal multiphoton microscope (MMM) based on laser amplitude splitting. An array of 8 _ 8 foci is created on the sample that gives a direct insight of the fluorescence localization. Four-dimensional (4D) imaging is obtained by combining simultaneous foci scanning, timegated detection, and z displacement. We illustrate time-resolved MMM capabilities for 4D imaging of a photosensitizer inside living colon cancer cells. The aim of this study is to have a better understanding of the photophysical processes implied in the photosensitizer reactivity
Document type :
Journal articles
Complete list of metadatas

Cited literature [23 references]  Display  Hide  Download
https://hal-iogs.archives-ouvertes.fr/hal-00691865
Contributor : Marie-Laure Edwards <>
Submitted on : Friday, April 27, 2012 - 11:19:24 AM
Last modification on : Thursday, January 11, 2018 - 6:26:16 AM
Long-term archiving on : Saturday, July 28, 2012 - 2:55:22 AM

File

P131.pdf
Publisher files allowed on an open archive

Identifiers

  • HAL Id : hal-00691865, version 1

Collections

LCF_LASBIO | LCFIO | IOGS | PARISTECH | LCF_LASERS

Citation

Ariane Deniset-Besseau, Sandrine Leveque-Fort, M.P. Fontaine-Aupart, Gérard Roger, Patrick Georges. Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells. Applied optics, Optical Society of America, 2007, 46 (33), pp.8045-8051. ⟨hal-00691865⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

255

Files downloads

192

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD