Total internal reflection fluorescence lifetime imaging microscope to probe FRET in neurobiology

Viviane Devauges (1, 2, 3) , Pierre Blandin (1, 2, 3) , J.C. Cossec (4) , Sandrine Lecart (2) , C. Marquer (4) , M.C. Potier (4) , Frédéric Druon (3) , Patrick Georges (3) , Sandrine Leveque-Fort (1, 2)

1 PPM - Laboratoire de Photophysique Moléculaire
2 CPBM - Centre de Photonique Biomédicale
3 Laboratoire Charles Fabry de l'Institut d'Optique / Elsa
4 CRICM - Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière

We present the development of a time resolved TIRF microscope illuminated by a supercontinuum laser source. It permits to perform wide-field fluorescence lifetime imaging of neurobiological processes at the plasma membrane with subwavelength axial resolution.

Domains: Physics [physics]; Physics [physics]; Optics [physics.optics]

Viviane Devauges, Pierre Blandin, J.C. Cossec, Sandrine Lecart, C. Marquer, et al.. Total internal reflection fluorescence lifetime imaging microscope to probe FRET in neurobiology. BIOS--Photonics West 2010, Jan 2010, xx, United States. ⟨hal-00618608⟩