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Total internal reflection fluorescence lifetime imaging microscope to probe FRET in neurobiology

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Abstract

We present the development of a time resolved TIRF microscope illuminated by a supercontinuum laser source. It permits to perform wide-field fluorescence lifetime imaging of neurobiological processes at the plasma membrane with subwavelength axial resolution.
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Dates and versions

hal-00618608 , version 1 (02-09-2011)

Identifiers

  • HAL Id : hal-00618608 , version 1

Cite

Viviane Devauges, Pierre Blandin, J.C. Cossec, Sandrine Lecart, C. Marquer, et al.. Total internal reflection fluorescence lifetime imaging microscope to probe FRET in neurobiology. BIOS--Photonics West 2010, Jan 2010, xx, United States. ⟨hal-00618608⟩
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