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Journal Articles Journal of Physical Chemistry Letters Year : 2020

Total correlation spectroscopy across all NMR-active nuclei by mixing at zero field

Abstract

Multidimensional Nuclear Magnetic Resonance (NMR) is based on the combination of well-established building blocks for polarization transfer. These blocks are used to design correlation experiments through one or a few chemical bonds or through space. Here, we introduce a building block that enables polarization transfer across all NMR-active nuclei in a coupled network of spins: isotropic mixing at Zero and Ultra-Low Field (ZULF). Exploiting mixing under ZULF-NMR conditions, heteronuclear TOtal Correlation SpectroscopY (TOCSY) experiments were developed to highlight coupled spin networks. We demonstrate 1 H-13 C and 1 H-15 N correlations in ZULF-TOCSY spectra of labelled amino acids, which allow one to obtain cross-peaks among all hetero-nuclei belonging to the same coupled network, even when the direct interaction between them is negligible. We also demonstrate the interest of ZULF-TOCSY to analyze complex mixtures on a growth medium of isotope-labelled biomolecules. ZULF-TOCSY enables the quick identification of individual compounds in the mixture by their coupled spin networks. The ZULF-TOCSY method will lead to the development of a new toolbox of experiments to analyze complex mixtures by NMR.
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Dates and versions

hal-03098498 , version 1 (05-01-2021)

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Ivan V Zhukov, Alexey S Kiryutin, Fabien Ferrage, Gerd Buntkowsky, Alexandra V Yurkovskaya, et al.. Total correlation spectroscopy across all NMR-active nuclei by mixing at zero field. Journal of Physical Chemistry Letters, 2020, 11 (17), pp.7291-7296. ⟨10.1021/acs.jpclett.0c02032⟩. ⟨hal-03098498⟩
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