Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

Arnaud Dubois 1 Olivier Levecq 1 Hicham Azimani 1 David Siret 1 Anaïs Barut Mariano Suppa Véronique Del Marmol Josep Malvehy Elisa Cinotti Pietro Rubegni Jean-Luc Perrot 2
1 Laboratoire Charles Fabry / Biophotonique
LCF - Laboratoire Charles Fabry
2 Valorial - Valorisation Recherche et Innovations Alimentaire
Abstract : An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo. LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection using a broadband laser and a line-scan camera. LC-OCT measures the echo-time delay and amplitude of light backscattered from cutaneous microstructures through low-coherence interferometry associated with confocal spatial filtering. Multiple A-scans are acquired simultaneously while dynamically adjusting the focus. The resulting cross-sectional B-scan image is produced in real time at 10 frame∕s. With an isotropic spatial resolution of ∼1 μm, the LC-OCT images reveal a comprehensive structural mapping of skin at the cellular level down to a depth of ∼500 μm. LC-OCT has been applied to the imaging of various skin lesions, in vivo, including carcinomas and melanomas. LC-OCT images are found to strongly correlate with conventional histopathological images. The use of LC-OCT as an adjunct tool in medical practice could significantly improve clinical diagnostic accuracy while reducing the number of biopsies of benign lesions.
Keywords : confocal microscopy optical coherence tomography skin cancer optical imaging
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Journal articles
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https://hal-iogs.archives-ouvertes.fr/hal-01913796
Contributor : Arnaud Dubois <>
Submitted on : Tuesday, November 6, 2018 - 3:30:59 PM
Last modification on : Friday, November 16, 2018 - 1:13:12 AM
Long-term archiving on : Thursday, February 7, 2019 - 3:02:45 PM

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Arnaud Dubois, Olivier Levecq, Hicham Azimani, David Siret, Anaïs Barut, et al.. Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors. Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2018, 23. ⟨hal-01913796⟩

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