LED-based full-field optical coherence microscopy for high-resolution in vivo imaging

Jonas Ogien 1 Arnaud Dubois 1
1 Laboratoire Charles Fabry / Biophotonique
LCF - Laboratoire Charles Fabry
Abstract : Full-field optical coherence microscopy (FF-OCM) is an imaging technique, based on low-coherence interference microscopy, that uses full-field illumination with a low-coherence light source and detection with an area camera to acquire en face tomographic images of semi-transparent samples. FF-OCM benefits from the transverse imaging resolution of optical microscopy along with the capacity of optical axial sectioning at micrometer-scale resolution. Since the introduction of FFOCM in the early 2000s, significant technological advances in this imaging modality have been achieved. FFOCM has been demonstrated in a variety of applications, in the biomedical field especially for cellular-level resolution imaging of biological tissues. This work reports on a FF-OCM setup specifically designed to meet the needs for high-resolution in vivo imaging, illuminated by a high-brightness broadband light emitting diode (LED). Broadband LEDs have spectra potentially large enough to provide imaging spatial resolutions similar to those reached using conventional halogen lamps, but their radiance can be much higher, which leads to high-speed acquisition and makes in vivo imaging easier. We introduce a FF-OCM setup using a 2.3 W broadband LED, with an interferometer designed to be as compact as possible in order to provide the basis for a portable system that will make it possible to fully benefit from the capacity for in vivo imaging by providing the ability to image any region of interest in real-time. The interferometer part of the compact FF-OCM setup weighs 210 g for a size of 11×11×5 cm3. Using this setup, a sub-micron axial resolution was reached, with a detection sensitivity of 68 dB at an imaging rate of 250 Hz. High resolution in vivo human skin images were obtained with this setup and compared with images of excised human skin, showing high similarity.
Type de document :
Communication dans un congrès
Journées Imagerie Optique Non-Conventionnelle, Mar 2017, Paris, France
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https://hal-iogs.archives-ouvertes.fr/hal-01521691
Contributeur : Arnaud Dubois <>
Soumis le : vendredi 12 mai 2017 - 10:36:17
Dernière modification le : mercredi 17 janvier 2018 - 13:34:02

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  • HAL Id : hal-01521691, version 1

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Jonas Ogien, Arnaud Dubois. LED-based full-field optical coherence microscopy for high-resolution in vivo imaging. Journées Imagerie Optique Non-Conventionnelle, Mar 2017, Paris, France. 〈hal-01521691〉

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