Spreading Dynamics of Biomimetic Actin Cortices - Archive ouverte HAL Access content directly
Journal Articles Biophysical Journal Year : 2011

Spreading Dynamics of Biomimetic Actin Cortices

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1
Michael Murrell
  • Function : Author
  • PersonId : 941106
Lea-Laetitia Pontani
Karine Guevorkian
Damien Cuvelier
  • Function : Author
  • PersonId : 941107
Pierre Nassoy
  • Function : Author
  • PersonId : 956882
C. Sykes
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Abstract

Reconstituted systems mimicking cells are interesting tools for understanding the details of cell behavior. Here, we use an experimental system that mimics cellular actin cortices, namely liposomes developing an actin shell close to their inner membrane, and we study their dynamics of spreading. We show that depending on the morphology of the actin shell inside the liposome, spreading dynamics is either reminiscent of a bare liposome (in the case of a sparse actin shell) or of a cell (in the case of a continuous actin shell). We use a mechanical model that qualitatively accounts for the shape of the experimental curves. From the data on spreading dynamics, we extract characteristic times that are consistent with mechanical estimates. The mechanical characterization of such stripped-down experimental systems paves the way for a more complex design closer to a cell. We report here the first step in building an artificial cell and studying its mechanics.
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Dates and versions

hal-00821326 , version 1 (09-05-2013)

Identifiers

  • HAL Id : hal-00821326 , version 1

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Michael Murrell, Lea-Laetitia Pontani, Karine Guevorkian, Damien Cuvelier, Pierre Nassoy, et al.. Spreading Dynamics of Biomimetic Actin Cortices. Biophysical Journal, 2011, 100 (6), pp.1400-1409. ⟨hal-00821326⟩
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