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Effets thérapeutiques des champs électriques pulsés nanosecondes sur modèle murin de métastates péritonéales coliques

Abstract : The aim of this study was to evaluate the sensitivity of murine colorectal cancer (CRC) CT-26 cells to 10 ns pulses, at high frequency and high intensity (nsPEF). We have developed a mouse model of colorectal peritoneal metastasis (PMc) and the histological response was then assessed according to a new classification (Peritoneal Regression Grading Score). Fibrosis was analyzed by multiphoton microscopy, proliferation (KI67, DAPI) and expression of immunological factors (CD3, CD4, CD8) by classical histology. We have demonstrated that nsPEFs display a dose-response effect on CT-26 cells by inducing cell death and plasma membrane permeabilization, as well as altering mitochondria morphology. In addition, a specific immune response (CD8+ T lymphocytes) and a major histological response have confirmed the potentiality of nsPEFs as an anti-tumor therapeutic alternative. We still need to evaluate the innocuity of nsPEFs on healthy peritoneum and the ergonomics of the application system are challenging to be improved.
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Submitted on : Sunday, October 10, 2021 - 1:01:31 AM
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Abdelkader Taibi. Effets thérapeutiques des champs électriques pulsés nanosecondes sur modèle murin de métastates péritonéales coliques. Médecine humaine et pathologie. Université de Limoges, 2020. Français. ⟨NNT : 2020LIMO0031⟩. ⟨tel-03372210⟩

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