Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish

Gabriela Certad (1, 2) , Alireza Zahedi (3) , Nausicaa Gantois (1) , Manasi Sawant (1) , Colette Creusy (2) , Erika Duval (2) , Sadia Benamrouz-Vanneste (1, 4) , Una Ryan (3) , Eric Viscogliosi (1)

1 CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204
2 GHICL - Groupement des Hôpitaux de l'Institut Catholique de Lille
3 Murdoch University
4 ICL - Institut Catholique de Lille

Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be genetic variants of the same species, while the designated novel marine genotype 2 (#Cryptofish 7) is clearly representative of a separate species.

Mots clés: genetic characterization; 18S rDNA gene; actin gene; molecular phylogeny; piscine Cryptosporidium; edible marine fish

Domaines: Parasitologie; Systématique, phylogénie et taxonomie

Gabriela Certad, Alireza Zahedi, Nausicaa Gantois, Manasi Sawant, Colette Creusy, et al.. Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish. Microorganisms, 2020, 8 (12), pp.2014. ⟨10.3390/microorganisms8122014⟩. ⟨hal-03086923⟩