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Journal Articles Optics Express Year : 2019

3D optical nanoscopy with excited state saturation at liquid helium temperatures

Abstract

We present a 3D fluorescence nanoscopy method operating at cryogenic temperatures, based on optical saturation of the excited state of individual molecules. Using a focused laser beam structured with a zero-intensity central region surrounded by intensity gradients in the three space directions, we achieve a sub-30 nm 3D optical resolution. Moreover, the analysis of the fluorescence scanning images of single molecules reveals the 3D orientation of their transition dipole with an accuracy of a few degrees. This method provides a valuable tool for locating neighboring molecules with overlapping optical transitions in order to study their interactions.
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Dates and versions

hal-02186046 , version 1 (17-07-2019)

Identifiers

  • HAL Id : hal-02186046 , version 1

Cite

J.-B Trebbia, R Baby, P. Tamarat, B. Lounis. 3D optical nanoscopy with excited state saturation at liquid helium temperatures. Optics Express, inPress. ⟨hal-02186046⟩
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