Skull optical clearing for longitudinal non invasive optical imaging

H Soleimanzad (1) , M Juchaux (2) , D Crepin (2) , H Gurden (1) , F Pain (3)

1 BFA (UMR_8251 / U1133) - Unité de Biologie Fonctionnelle et Adaptative
2 IJCLab - Laboratoire de Physique des 2 Infinis Irène Joliot-Curie
3 Laboratoire Charles Fabry / Biophotonique

We have evaluated in vitro and in vivo the efficiency and practicability of a recently proposed approach for minimally invasive, longitudinal imaging of the rodent brain: reversible optical clearing of the skull 1. Use of this approach for rodent brain imaging would allow to suppress the need of time consuming and prone to defect chronic optical windows 2,3. In addition, the method would allow to preserve completely the dura matter and brain tissues thus unaltering the physiology of the tissues. Firstly, in vitro experiments have been conducted on resected mice skulls to determine whether a two steps protocol including calcium chelation followed by topical application of an index matching solution is better than solely the direct optical index matching strategy 4. The time course of the effect of different chemicals on the optical properties of the skull have been monitored quantitative to provide with an optimal protocol for in vivo skull optical clearing. Secondly, in vivo validation was carried out for mapping of brain blood flow using multiple exposure laser speckle imaging.

Mots clés: clearing; skull; longitudinal imaging; laser speckle imaging; cerebral blood flow; mice; brain

Domaines: Optique / photonique; Neurosciences

H Soleimanzad, M Juchaux, D Crepin, H Gurden, F Pain. Skull optical clearing for longitudinal non invasive optical imaging. SPIE West BIOS 2020, Feb 2020, San Francisco, United States. ⟨hal-03066940⟩