Simultaneous optically sectioned fluorescence and optical coherence microscopy with full-field illumination

Houssine Makhlouf (1) , Karen Perronet (1) , Guillaume Dupuis (2) , Sandrine Leveque-Fort (3) , Arnaud Dubois (1)

1 Laboratoire Charles Fabry / Biophotonique
2 CPBM - Centre de Photonique Biomédicale
3 ISMO - Institut des Sciences Moléculaires d'Orsay

Full-field optical coherence microscopy (FF-OCM) and optically sectioned fluorescence microscopy are two ima- ging techniques that are implemented here in a novel dual modality instrument. The two imaging modalities use a broad field illumination to acquire the entire field of view without raster scanning. Optical sectioning is achieved in both imaging modalities owing to the coherence gating property of light for FF-OCM, and a structured illumination setup for fluorescence microscopy. Complementary image data are provided by the dual modality instrument in the context of biological tissue screening. FF-OCM imaging modality shows the tissue microarchitecture, while fluor- escence microscopy highlights specific tissue features with cellular-level resolution by using targeting contrast agents. Complementary tissue morphology and biochemical features could potentially improve the understanding of cellular functions and disease diagnosis.

Domaines: Optique [physics.optics]; Biophysique [physics.bio-ph]

Houssine Makhlouf, Karen Perronet, Guillaume Dupuis, Sandrine Leveque-Fort, Arnaud Dubois. Simultaneous optically sectioned fluorescence and optical coherence microscopy with full-field illumination. Optics Letters, 2012, 37, pp.1613. ⟨hal-00694961⟩